|
纳米粒子广泛用于医学诊断，特别是黄金纳米粒子（AuNPs），由于其特殊的光学特性，更是广泛地作为生物传感器运。当AuNPs 溶液中存在PNA时，由于PNA是中性电荷的，PNA 起到了凝集剂的作用，促使AuNPs 聚合。 当AuNPs聚合时，AuNPs粒子会发生光学变化，如颜色改变（红色变成暗紫色）。利用这个原理，可以检测样品中是否存在某一特定核酸序列，从而检测是否有相应的病原体。
如果溶液中存在跟PNA互补的RNA片段，则PNA会跟RNA配对。这时PNA就不能促使AuNPs 凝集，颜色不会变成暗紫色。
1 PNA单独存在时，能够促使AuNP 凝集（暗紫色）。
2 当存在与PNA互补的RNA时，PNA与RNA结合，AuNP处于分散状态（红色）
3 当溶液中的RNA与PNA不互补时，PNA不会跟RNA特异性结合，AuNP还是会凝集（暗紫色）
-
Kanjanawarut R, Su X. Colorimetric detection of DNA using unmodified metallic nanoparticles and peptide nuclei acid probes. Anal Chem. 2009;81:6122–6129. doi: 10.1021/ac900525k. [PubMed] [Cross Ref]
-
Lehtola MJ, Loades CJ, Keevil CW. Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari. J Microbiol Methods. 2005;62:211–219. doi: 10.1016/j.mimet.2005.02.009.[PubMed] [Cross Ref]
-
Li H, Rothberg L. Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. PNAS. 2004;101:14036–14039. doi: 10.1073/pnas.0406115101. [PMC free article] [PubMed] [Cross Ref]
-
Nielsen PE, Egholm M. An introduction to peptide nucleic acid. Curr Issues Mol Biol. 1999;1:89–104. [PubMed]
-
Paulasova P, Pellestor F. The peptide nucleic acids (PNAs): a new generation of probes for genetic and cytogenetic analyses. Ann Genet. 2004;47:349–358. doi: 10.1016/j.anngen.2004.07.001. [PubMed] [Cross Ref]
-
Pinior B, Firth CL, Richter V, Lebl K, Trauffler M, Dzieciol M, Hutter SE, Burgstaller J, Obritzhauser W, Winter P, Käsbohrer A. A systematic review of financial and economic assessments of bovine viral diarrhea virus (BVDV) prevention an mitigation activities worldwide. Prev Vet Med. 2017;137:77–92. doi: 10.1016/j.prevetmed.2016.12.014. [PubMed] [Cross Ref]
-
Pita M, Abad JM, Vaz-Dominguez C, Briones C, Mateo-Martí E, Martín-Gago JA, Morales Mdel P, Fernández VM. Synthesis of cobalt ferrite core/metallic shell nanoparticles for the development of a specific PNA/DNA biosensor. J Colloid Interface Sci. 2008;321:484–492. doi: 10.1016/j.jcis.2008.02.010. [PubMed][Cross Ref]
-
Ridpath JF, Passler T. Editorial: control of Pestivirus infections in the management of wildlife populations. Front Microbiol. 2016;7:1396. [PMC free article] [PubMed]
【打印此文】【关闭窗口】 |
